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Molecular Biology
A Guide to the NGS Workflow
NGS Library Preparation and Sample Prep
KAPA Pure Beads
KAPA Pure Beads from Roche
Be the first to write a review!
Citations:
(236)
Supplier Page
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Roche for
KAPA Pure Beads
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Product Specs
Item
KAPA Pure Beads
Company
Roche
Price
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Catalog Number
07983280001
Quantity
30 mL
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Roche
Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road
Indianapolis
,
Indiana
United States
Phone
: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911
Company Profile
Website:
sequencing.roche.com
Citations (236)
(81)
Genomeâ€wide prediction of activating regulatory elements in rice by combining STARR â€seq with FACS
Plant Biotechnology Journal
August 26, 2022
Wei Tian, Xi Huang, Xinhao Ouyang
preparation Nip genomic DNA was sonicated using a Bioruptor (Diagenode). Fragments were recovered using 0.6X
KAPA Pure Beads
(
KAPA
) and subjected to a
KAPA
Hyper Prep
Kit (KAPA) to add consensus adapters following the manufacturer's
(82)
COL17A1 editing via homology-directed repair in junctional epidermolysis bullosa
Frontiers in Medicine
August 25, 2022
Igor Petković et al.
and include adapter sequences for index-PCRs further downstream. These PCRs were then purified with
Kapa Pure Beads
(
Roche
, Basel, Switzerland) following the manufacturer’s protocol.
PCR
fragments below 200 bp were excluded
(83)
Draft Genome Sequence of Pseudomonas sp. Strain MWU15-20650, Isolated from Wild Cranberry Fruit in the Cape Cod National Seashore
Microbiology Resource Announcements
August 18, 2022
Tyler Sholl, Scott Soby
Integrated DNA Technologies) were individually ligated to each sample, the samples were cleaned with
KAPA pure beads
(KK8002;
Kapa Biosystems
) and amplified with HiFi enzyme (KK2502; Kapa Biosystems). Fragment sizes were
(84)
Draft Genome Sequence of Pseudomonas protegens Strain MWU12-2233, Isolated from Wild Cranberry Fruit in Provincetown, Massachusetts
Microbiology Resource Announcements
August 18, 2022
Michelle Wood, Scott D. Soby
(Integrated DNA Technologies; 00989130v2) were ligated individually to each sample, followed by cleaning with
Kapa pure beads
(
Kapa
Biosciences; KK8002), and amplified with a HiFi enzyme (KK2502). Fragment size was determined
(85)
Essential Roles of the Histone Demethylase KDM4C in Renal Development and Acute Kidney Injury
International journal of molecular sciences
August 18, 2022
Pan HC, Chen YH, Fang WC, Wu VC, Sun CY.
Roche
, Basel, Switzerland), along with
library amplification
primers.
PCR
products were purified using a
KAPA Pure Beads
system, and the library quality was assessed using the Qsep 100 DNA/RNA Analyzer (BiOptic Inc., New
(86)
Integrated analysis of small RNAs, transcriptome and degradome sequencing reveal the drought stress network in Agropyron mongolicum Keng
Frontiers in Plant Science
August 18, 2022
Bobo Fan et al.
United States). The attached product was transferred into a new centrifuge tube and supplemented with
KAPA Pure Beads
(kk8000;
Roche Diagnostics
Application, Indianapolis, IN, United States). The reverse transcription
(87)
Ultra Deep Sequencing of Circulating Cell-Free DNA as a Potential Tool for Hepatocellular Carcinoma Management
Cancers (Basel)
August 11, 2022
Mónica Higuera et al.
MiSeq platform (Illumina, San Diego, CA, USA),
amplification
products were pooled and purified using
Kapa pure beads
(
Roche Applied Science
, Penzberg, Germany). Pools were quantified by Qubit (ThermoFisher, Waltham, MA,
(88)
Heterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
International Journal of Molecular Sciences
August 5, 2022
Andrey Valentinovich Zadorozhny et al.
alkaline phosphatase 16 °C for 120 min. Obtained DNA fragments with sticky ends were purified using
KAPA Pure Beads
(
Roche
, Basel, Switzerland), according to the manufacturer’s instructions, and were used in
ligation
(89)
Paired nicking-mediated COL17A1 reframing for junctional epidermolysis bullosa
Molecular Therapy
August 1, 2022
Johannes Bischof, Oliver Patrick March, Bernadette Liemberger, Simone Alexandra Haas, Stefan Hainzl, Igor Petković, Victoria Leb-Reichl, Julia Illmer, Evgeniia Korotchenko, Alfred Klausegger,
et al.
from cDNA and DNA of nucleofected cells (Table S6). These amplicons were subsequently purified with
Kapa Pure Beads
(
Roche
, Basel, Switzerland) according to the manufacturer’s protocol. To remove primer dimers, fragments
(90)
Deep Sequencing Analysis of Individual HIV-1 Proviruses Reveals Frequent Asymmetric Long Terminal Repeats
Journal of Virology
July 13, 2022
Kevin W. Joseph, Elias K. Halvas, Leah D. Brandt, Sean C. Patro, Jason W. Rausch, Abha Chopra, Simon Mallal, Mary F. Kearney, John M. Coffin, John W. Mellors
followed by 65°C for 10 min, and then held at 4°C. After MDA, the plate was purified by adding 20 μL
Kapa pure beads
(
Kapa Biosystems
, Wilmington, MA, USA) according to the manufacturer’s recommendations, including
incubation
(91)
Metagenomic Investigation of Ticks From Kenyan Wildlife Reveals Diverse Microbial Pathogens and New Country Pathogen Records
Frontiers in Microbiology
July 1, 2022
Koray Ergunay, Mathew Mutinda, Brian Bourke, Silvia A. Justi, Laura Caicedo-Quiroga, Joseph Kamau, Samson Mutura, Irene Karagi Akunda, Elizabeth Cook, Francis Gakuya,
et al.
(Agilent Technologies, VA, United States). Excess adapter dimer and small fragments were removed using
KAPA pure beads
(
Roche
, CA, United States). Unbiased metagenomic
sequencing
was performed on the NovaSeq platform (Illumina,
(92)
Molecular landscapes of human hippocampal immature neurons across lifespan
Nature
July 1, 2022
Zhou Y, Su Y, Li S, Kennedy BC, Zhang DY, Bond AM, Sun Y, Jacob F, Lu L, Hu P,
et al
.
of EvaGreen dye (Biotium, 31000) for a
qPCR
thermocycling. The
PCR
products were then purified using
KAPA Pure Beads
(
KAPA Biosystems
, KK8000). One hippocampal specimen was processed using droplet-based snRNA-seq technique56
(93)
TBX2 acts as a potent transcriptional silencer of tumour suppressor genes through interaction with the CoREST complex to sustain the proliferation of breast cancers
Nucleic acids research
June 24, 2022
McIntyre AJ, Angel CZ, Smith JS, Templeman A, Beattie K, Beattie S, Ormrod A, Devlin E, McGreevy C, Bothwell C,
et al
.
manufacturer's instructions. Libraries were size-selected to an average fragment size of 300-400 bp using
KAPA Pure Beads
(
Roche
) which was confirmed with an Agilent High
Sensitivity
DNA Kit (Agilent Technologies) following
(94)
Bisulfite-free and single-nucleotide resolution sequencing of DNA epigenetic modification of 5-hydroxymethylcytosine using engineered deaminase†
Chemical Science
June 15, 2022
Neng-Bin Xie, Min Wang, Tong-Tong Ji, Xia Guo, Jiang-Hui Ding, Bi-Feng Yuan, Yu-Qi Feng
DNA was treated with β-GT (New England Biolabs) at 37 °C for 2 h followed by
purification
using 0.9 ×
KAPA pure beads
(
Roche
). The resulting DNA (60 ng) was denatured and treated with 20 μM of wtA3A. The deaminase-treated
(95)
SARS-CoV-2 Mutant Spectra at Different Depth Levels Reveal an Overwhelming Abundance of Low Frequency Mutations
Pathogens
June 8, 2022
Brenda Martínez-González et al.
concentration of the
RT-PCR
products was adjusted to 4 × 109 molecules/µL, and DNA pools were purified using
Kapa Pure Beads
(
Kapa Biosystems
, Roche). Purified DNA was quantified using Qubit (Qubit dsDNA
Assay
kit, Thermofisher
(96)
Dynamics of Salmonella enterica and antimicrobial resistance in the Brazilian poultry industry and global impacts on public health
PLOS Genetics
June 2, 2022
Nabil-Fareed Alikhan et al.
equal quantities. The final pool was double-SPRI size selected between 0.5 and 0.7X bead volumes using
KAPA Pure Beads
(
Roche
Catalogue No.
07983298001
,
Roche
, Basel, Switzerland). The final pool was quantified on a Qubit
(97)
Metabarcoding of Parasitic Wasp, Dolichogenidea metesae (Nixon) (Hymenoptera: Braconidae) That Parasitizing Bagworm, Metisa plana Walker (Lepidoptera: Psychidae)
Tropical Life Sciences Research
June 2, 2022
Aqilah Sakinah Badrulisham et al.
products were checked on a 1.5% of agarose gel. The DNA is then purified using 0.7× volume ratio of
KAPA pure beads
(
KAPA Biosystems
, USA). Second
PCR
step for index tagmentation were done using Illumina Nextera XT Index
(98)
JAZF1-SUZ12 dysregulates PRC2 function and gene expression during cell differentiation
Cell reports
May 31, 2022
Tavares M, Khandelwal G, Muter J, Viiri K, Beltran M, Brosens JJ, Jenner RG.
mg/mL RNaseA and 0.2 mg/mL of
proteinase K
(
Ambion
,
AM2546
). DNA was purified from this aliquot using
KAPA Pure beads
(
Roche
, KK8000), resuspended in 10 mM
Tris
pH 8 and quantified using the
Qubit dsDNA HS Assay kit
(
Thermofisher
,
(99)
A systematic dissection of determinants and consequences of snoRNA-guided pseudouridylation of human mRNA
Nucleic Acids Research
May 20, 2022
Ronit Nir, Thomas Philipp Hoernes, Hiromi Muramatsu, Klaus Faserl, Katalin Karikó, Matthias David Erlacher, Aldema Sas-Chen, Schraga Schwartz
fragment was cut from a gel, purified by Qiaquick gel extraction kit (Qiagen) and cleaned from salts using
Kapa pure beads
(
Roche
). 2 pmol DNA was used for
in vitro transcription
, carried out using T7 Megascript transcription
(100)
Microbiota Composition of Mucosa and Interactions between the Microbes of the Different Gut Segments Could Be a Factor to Modulate the Growth Rate of Broiler Chickens
Animals (Basel)
May 18, 2022
Valéria Farkas, Gábor Csitári, László Menyhárt, Nikoletta Such, László Pál, Ferenc Husvéth, Mohamed Ali Rawash, Ãkos MezÅ‘laki, Károly Dublecz
mg samples using the AquaGenomic Kit (MoBiTec GmbH, Göttingen, Germany) and further purified using
KAPA Pure Beads
(
Roche
, Basel, Switzerland) according to the manufacturer’s protocols. The concentration of genomic
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KAPA Pure Beads from Roche
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